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If generating v2 sample sheets for devices besides the ones detailed, verify the ultimate sample sheet has the i5 index in the proper orientation as described over.
Take a look at how AI is reworking genomic exploration, driving medical insights, and enabling multiomic discovery
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Seek advice from Index 2 (i5) Orientation To learn more regarding how to enter i5 bases around the sample sheet in forward or reverse enhance orientation.
When demultiplexing, the ultimate sample sheet furnished to the FASTQ era software program ought to have the i5 index in the proper orientation for that System. Nevertheless, most Illumina sample sheet era application handles this mechanically.
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NextSeq 1000/2000 and NovaSeq X/X Furthermore i5 indexes will appropriately be output inside the forward orientation for afterwards downstream processing. Tailor made kits also needs to provide the i5 indexes entered while in the forward orientation.
Examine how AI is reworking genomic analysis, driving clinical insights, and enabling multiomic discovery
When working with BaseSpace Sequence Hub PrepTab, i5 sequences has to be entered into these software from the forward orientation in all situation and for all instrument kinds.
For runs uploaded manually (i.e., an Illumina Device is just not getting used to crank out the sample sheet), the index orientation relies on which instrument you are using. Please see the Index Orientation Desk for info on tips on how to enter the i5 sequence depending on the instrument and software used.